A Key Study on Pollen-Specific SFB Genotype and Identification of Novel SFB Alleles from 48 Accessions in Japanese Apricot (Prunus mume Sieb. et Zucc.)

Self-incompatibility (SI) is a common strategy to avoid inbreeding and, consequently, keep genetic diversity within species. In its mechanism, pollen rejection happens in the style when single multiallelic locus (SFB prunus species) of haploid matches one S-alleles existing diploid pistil. The SFB gene for S has been identified many Prunus However, Japanese apricot species with typical gametophytic self-incompatibility (GSI), and alleles available are limited, although they required studying GSI. Therefore, we used an AS-PCR amplification method, sequencing, pair primers SFB-C1F Pm-Vb designed based on conserved region identify genotypes 48 (P. mume) accessions. Eleven novel were isolated from these accessions shared structural features other These uniquely expressed pollen. Hence, concluded that 11 PmSFB determinants P. mume. This current study offers genes mume locus, which could be useful potential resource studies SI mechanisms.